Transformation of Corynespora cassiicola by Agrobacterium tumefaciens
نویسندگان
چکیده
منابع مشابه
Agrobacterium tumefaciens-mediated transformation of yeast.
Agrobacterium tumefaciens transfers a piece of its Ti plasmid DNA (transferred DNA or T-DNA) into plant cells during crown gall tumorigenesis. A. tumefaciens can transfer its T-DNA to a wide variety of hosts, including both dicotyledonous and monocotyledonous plants. We show that the host range of A. tumefaciens can be extended to include Saccharomyces cerevisiae. Additionally, we demonstrate t...
متن کاملTransformation of Arabidopsis thaliana with Agrobacterium tumefaciens.
Transformed Arabidopsis thaliana plants have been produced by a modified leaf disk transformation-regeneration method. Leaf pieces from sterilely grown plants were precultured for 2 days and inoculated with an Agrobacterium tumefaciens strain containing an avirulent Ti (tumor-inducing) plasmid with a chimeric gene encoding hygromycin resistance. After cocultivation for 2 days, the leaf pieces w...
متن کاملAgrobacterium tumefaciens- mediated Transformation of Plant Cells
Agrobacterium tumefaciens is a Gram-negative soil bacterium that causes plant tumours by transferring a portion of DNA from a resident ‘tumour inducing’ (Ti) plasmid into plant cells where it is integrated into a plant chromosome and expressed. This bacteria’s capacity for DNA transfer is the basis of most current plant genetic engineering and make it a model system for the study of pathogenic ...
متن کاملGenetic transformation of Robinia pseudoacacia by Agrobacterium tumefaciens.
Transgenic Robinia pseudoacacia plants were obtained by Agrobacterium tumefaciens mediated gene transfer. Agrobacterium strain LBA4404 harbouring a binary vector that contained the chimeric neomycin phosphotransferase II (NPTII) and beta-glucuronidase (GUS) genes was co-cultivated with hypocotyl segments of in vitro raised seedlings of Robinia. Parameters important for high efficiency regenerat...
متن کاملProgress of cereal transformation technology mediated by Agrobacterium tumefaciens
Monocotyledonous plants were believed to be not transformable by the soil bacterium Agrobacterium tumefaciens until two decades ago, although convenient protocols for infection of leaf disks and subsequent regeneration of transgenic plants had been well established in a number of dicotyledonous species by then. This belief was reinforced by the fact that monocotyledons are mostly outside the ho...
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ژورنال
عنوان ژورنال: Fungal Biology
سال: 2019
ISSN: 1878-6146
DOI: 10.1016/j.funbio.2019.05.011